Modul: Enzyme Technology (1502-510)

Personen:

• Prof. Dr. Lutz Fischer (verantwortlich)

Studiengang:

• Biologie (Master, PO vom 01.10.2010)
  3. Semester, Wahlpflicht
• Food Biotechnology (Master, PO vom 01.10.2016)
  3. Semester, Wahl

Bezug zu anderen Modulen:

The module is part of the series "Enzyme Biotechnology".

Sprache:

deutsch/englisch

ECTS:

7,5 credits

Angebotshäufigkeit:

jedes WS

Dauer des Moduls:

4 Wochen (Block 4)

Studienleistung:

Lab book - Attendance on the lab course -

Modulprüfung:

Oral presentation of results and discussion (50 %) - protocol (50%

Prüfungsdauer:

30 Minuten

Arbeitsaufwand:

90 h attendance + 135 h independent study = 225 h workload

Fachkompetenzen:

Upon completion of this modul, the students are able to design strategies to purify enzymes by Fast Protein Liquid Chromatography and to perform side directed mutagenesis techniques. Further, the students can evaluate the purification based on yield, purification factor and electrophoretic methods. After this modul the students can determine the enzyme activity of different enzymes using different methods (e.g. spectrometric, HPLC). Students have knowledge about different immobilisation methods of enzymes after this modul and can performe and evaluate a covalent immobilisation method on macropourus particels. Upon this modul, the students can perfom and evaluate biotransformation processes.

Schlüsselkompetenzen:

Upon completion of this module the students are able to plan and work in a laboratory independently. They will be able to interpret their results and to compare them with known data from literature. In addition, they will be able to present and discuss their results in the frame of an exam.

Anmerkungen:

Number of participants is limited to about 10 students because of limited lab space and instruments

eLearning:

Veranstaltung im ILIAS

Lehrveranstaltungen