Module: Protein Expression in Bacteria (2501-440)

This module not offered anymore!

Persons:

• Prof. Dr. Andreas Kuhn (verantwortlich)

Degree Program:

• Biology (Master, since 01.10.2010)
  1. Semester, semi-elective
• Food Biotechnology (Master, since 01.10.2013)
  3. Semester, elective
• Food Biotechnology (Master, since 01.10.2016)
  3. Semester, elective

Prerequisites for Attendance:

Basics of Protein Expression (B. Lewin: Gene Expression)

Sprache:

English

ECTS:

7,5 credits

Frequency:

every winter semester

Length of the Module:

4 weeks (block 2)

Module examination:

Written exam

Length of the examination:

60 minutes

Workload:

84 h attendance + 116 h independent study = 200 h workload

Professional competences:

The students will learn
- to design oligonucleotide primers for amplifying/modifying a bacterial gene using bioinformatics tools.
- how to modify a gene to enable its expression in E.coli using molecular biology methods.
- which parameters can be optimised for protein over-expression in bacteria using biochemical methods.
- to analyse, describe and present their data obtained and discussing them in the context of previous results from scientific publications.

Key competences:

The students will gain
- experience in planning and organising a scientific experiment.
- more confidence in performing experiments independently.
- more insights into a critical analysis of their data obtained.
- more familiarity with presenting and discussing data in English.

Comments:

Maximum number of participants: 10
Registration for participation: Letter of motivation and interview

eLearning:

Course in ILIAS

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