Course: Protein Expression in Bacteria (2501-441)

Persons:

• Prof. Dr. Andreas Kuhn (verantwortlich)
• Dr. rer. nat. Domenico Lupo (begleitend)

Type of Course:

lecture with exercise and seminar

In-Class Hours Per Week:

4

Contents:

RNA polymerase and ribosome function, transcription and translation, mRNA and protein degradation, promoter and ribosome binding, chaperones

Techniques: Site-directed mutagenesis, PCR, analytical restriction digest, DNA sequencing, SDS PAGE, Western blot

Literature:

B. Lewin: Genes, Oxford

Location:

Hohenheim

Module:

• 2501-440 Protein Expression in Bacteria (compulsory)

eLearning:

Course in ILIAS