Module: Molecular Genetics (2401-230)

Persons:

• Prof. Dr. Anette Preiss (verantwortlich)

Degree Program:

• Agricultural Biology (Bachelor, since 01.10.2015)
  6. Semester, elective
• Biologie Lehramt an Gymnasien (Master, since 01.10.2017)
  2. Semester, elective
• Biologie Lehramt an Gymnasien (Master, since 01.10.2017)
  4. Semester, elective
• Biologie Lehramt an Gymnasien | Erweiterungsmaster (Master, since 01.10.2017)
  4. Semester, elective
• Agricultural Biology (from WS 17/18 on) (Bachelor, since 01.10.2017)
  6. Semester, elective
• Agricultural Biology (from WS 18/19 on) (Bachelor, since 01.04.2018)
  6. Semester, elective
• Biology (Bachelor, since 01.04.2011)
  6. Semester, semi-elective

Prerequisites for Attendance:

Knowledge equivalent to the module "Genetics"

Sprache:

German

ECTS:

6 credits

Frequency:

every summer semester

Length of the Module:

blocked (by arrangement)

Compulsory assignment:

Regular and active participation, seminar talk and colloquium covering the topics of the seminar, 2-hour colloquium covering the topics of the practical training, own presentation of the exercises, written protocol (practical training)

Module examination:

Written exam covering the topics of the lecture and seminar

Length of the examination:

120 minutes

Workload:

113 h presence + 67 h personal contribution = 180 h workload

Professional competences:

Lecture:
The lecture covers basic molecular techniques including vectors and cloning strategies, promoter analyses, DNA-Protein interactions, Biocomputing for the analysis of sequences, genes and genomes, hybrid dysgenesis, generation of transgenic organisms, principles of transgenes and their application, legislature for the safe handling of genetically modified organisms (MGO).

Seminar:
Readings of current methods in molecular genetics; solutions of problems in molecular genetics in small groups with the help of bioinformatic tools

Practical course:
- Safety procedures and good laboratory standards in the molecular genetics lab
- Gene mapping, Southernblot, stringency, detection
- Transformation of bacteria, competence, efficiency, selection
- Expression vectors and constructs, bacterial protein expression, purification of fusion proteins by chromatography, PAGE
- Genotyping of transgenic lines, PCR, primer selection, diagnostic
- Protein-protein interactions in the yeast two-hybrid system
- Immunohistochemical detection of ectopic protein induction
- Gene manipulation: tissue specific overexpression of transgenes
- Temporally and spatially restricted RNA interference for knock down of gene acticity
- Quality management in the design and execution of molecular biology experiments (controls and documentation)

Comments:

Maximum number of participants: 12

eLearning:

Course in ILIAS

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