Course: Enzyme Technology, Practical Course (1502-512)

Persons:

• Prof. Dr. Lutz Fischer (verantwortlich)
• PD Dr. Timo Stressler (verantwortlich)

Type of Course:

practical

In-Class Hours Per Week:

4.5

Contents:

The students will perform different enzyme purification methods by their own. This includes primary purification methods like ammounium sulfate precipitation and different column chromatographic principles like ion exchange or hydrophobic interaction. The purification procedure will be evaluated by the students using different electrophoretic methods like SDS- and native-PAGE and the preparation of a purification table. Further, the students will perform different methods for enzyme activity measurments and learn how to biochemically characterize enzymes (e.g. pH, temperature, substrat specificity). Additionally, the students perform a covalent enzyme immobilisation and use the free and immobilised enzyme for biotransformation experiments. Finally, the students will design and evaluate a continuous biotransformation by their own.

Literature:

Current literature about particular purification protocol, original articles and reviews (will be discussed with supervisor).
Illanes, A.: Enzyme Biocatalysis, 1. ed., Springer, New York, 2008

Scopes, R. K.: Protein Purification Principles and Practice, 3. ed., Springer, New York, 1994

Location:

Hohenheim

Module:

• 1502-510 Enzyme Technology (compulsory)

eLearning:

Course in ILIAS