Lehrveranstaltung: Mutagenesis and Overexpression, Lecture (1502-481)

Personen:

• Prof. Dr. Lutz Fischer (verantwortlich)
• Dr. Sabine Lutz-Wahl (begleitend)

Lehrform:

Vorlesung

SWS:

1

Inhalt:

- Deepening of molecular biological methods for cloning of genes: DNA isolation methods, PCR, transformation methods, DNA se-quencing
- Introduction in sequence data libraries and software-tools for applied molecular biology
- Overexpression of enzymes: discussion of different expression systems (e.g. induction, stability of expression systems): E. coli, Lactobacillus, yeasts and their influences on enzyme production (yield), concepts of “food-grade”-systems, legal requirements in enzyme production for the food industry
- Methods for side-directed and error-prone mutagenesis
- Application examples for Directed Evolution and rational protein design

Literatur:

Brown, T.A.: Gene cloning and DNA analysis, 6. Auflage, John Wiley and Sons, 2010
Glick, B. R., Pasternak, J.J.: Molecular Biotechnology - Principles and application of recombinant DNA, 3. Auflage, ASM, Washington, 2003.
Gellissen, G.: Production of recombinant proteins, Wiley-VCH, Wein-heim, 2005.
current scientific publications (will be provided)

Veranstaltungsort:

Hohenheim

Modul:

• 1502-480 Mutagenesis and Overexpression of Enzymes (Pflicht)