Module: Methods in Molecular Biology and Biotechnology (3302-500)

Persons:

• Prof. Dr. Uwe Ludewig (verantwortlich)
• Prof.Dr. Artur Pfitzner (begleitend)
• PD Dr. Wolfgang Beyer (begleitend)
• Prof. Dr. Ralf Vögele (begleitend)
• Dr. Benjamin Neuhäuser (begleitend)
• Dr. Tobias Link (begleitend)
• PD Dr. rer. nat. Stefan Scholten (begleitend)

Degree Program:

• Crop Sciences - Plant Nutrition and Protection (Master, since 01.10.2014)
  1. Semester, compulsory

Relation to other Modules:

From Genes to transgenic plants (3503-450) Physiology and biochemistry of crops (3302-440) Molecular plant nutrition (3302-480) Molecular Phytopathology (3601-460) Molecular Plant Genetics (3503-480)

Prerequisites for Attendance:

Basics in molecular biology and genetics

Sprache:

English

ECTS:

12 credits

Frequency:

every winter semester

Length of the Module:

1 semester

Compulsory assignment:

Presentation (40 %) with discussion (10 %); compulsory attendance at laboratory training

Final examination:

Written paper in the form of a laboratory report in groups (50 %)

Workload:

224 h presence + 96 h preparation at home + exam = 320 h workload

Professional competences:

After successfully completing the module, students are able to outline diagnostics, production, selection and verification of transgenic plants, transgenic model plants (Arabidopsis) and crops. They can illustrate their interaction with microbial pathogens, their gene regulation, protein regulation, protein purification and manipulation of nutrient uptake. Students are proficient in basic laboratory skills, i.e. PCR, gene cloning, isolation and verification of mutant lines and protein purification.

Key competences:

Students enhance their organizational skills, self-reliance, time management and team work during exercises, while preparing and following up on lectures and while compiling their written protocols (laboratory report). They become more confident in laboratory work, while also linking their theoretical knowledge to practical experience in the laboratory. While writing the protocols, students improve their scientific articulateness and further improve their oral communication skills, presentation techniques and discourse capacities through presenting their work.

Comments:

The number of participants is limited to 20. Attendance compulsatory (max. 3 days excused absence is possible)

eLearning:

Course in ILIAS

Courses