Module: General Genetics II (2401-220)

Persons:

• Prof. Dr. Anette Preiss (verantwortlich)

Degree Program:

• Biologie Lehramt an Gymnasien (Master, since 01.10.2017)
  1. Semester, elective
• Biologie Lehramt an Gymnasien (Master, since 01.10.2017)
  3. Semester, elective
• Biologie Lehramt an Gymnasien | Erweiterungsmaster (Master, since 01.10.2017)
  3. Semester, elective
• Biology (Bachelor, since 01.04.2011)
  5. Semester, semi-elective

Relation to other Modules:

This module forms together with the modules "General Genetics I" and "General Virology" the elective profile Genetics for the study programme "Biology"

Prerequisites for Attendance:

Knowledge equivalent to the module "Genetics"

Sprache:

German

ECTS:

6 credits

Frequency:

every winter semester

Length of the Module:

blocked

Compulsory assignment:

written protocol covering the topics of the practical part of the exercises; oral presentation

Module examination:

written exam

Final examination:

written exam

Length of the examination:

90 minutes

Workload:

70 h presence + 110 h personal contribution = 180 h workload

Professional competences:

- Preparation and analysis of mammalian chromosomes, karyogram (phase contrast light microscopy)
- Temporally controlled induction of transgenes (heat shock puffs)
- Genetic fingerprint on the participants saliva
- Mutation and complementation using Drosophila mutants (light microscopy)
- In situ protein localization using immunochemistry: Drosophila neurogenesis (fluorescence microscopy)
- Preparation of Drosophila embryonic cuticles, analysis of neurogenic phenotypes (phase contrast light microscopy)
- Enhancer analysis with lacZ-reporter lines in Drosophila embryos (light microscopy)
- In vivo demonstration of gene activity: tissue specific overexpression of GFP in Drosophila imaginal discs; preparation of the tissue and fluorescence microscopy
- Quality management in the design and execution of molecular biology experiments (controls and documentation)

Key competences:

Ziel des Moduls ist, dass die Studierenden nach dessen Abschluss
- Übung in guter Laborpraxis und Sicherheitsaspekten im Bio-Labor haben
- die Dokumentation genetischer Experimente beherrschen
- die Wege zur Qualitätssicherung beim genetischen Experimentieren wissen

Comments:

Maximum number of participants: 16

eLearning:

Course in ILIAS

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