Course: Recombinant Proteins, Practical (1506-433)

Persons:

• Prof. Dr. Ralf Kölling-Paternoga (verantwortlich)

Type of Course:

practical

In-Class Hours Per Week:

2

Contents:

The following experiments will be performed:

- detection of a gene cassette insertion into the yeast genome by PCR
- agarose gel electrophoresis
- localization of cellulase activities in yeast by cell fractionation
- SDS-PAGE
- Western Blotting
- affinity-purification of 6His-tagged proteins from E. coli

Location:

Hohenheim

Remarks:

Molecular Biology of the Cell, Alberts, Wiley-VCH (2007)

Module:

• 1506-430 Recombinant Proteins (compulsory)

eLearning:

Course in ILIAS