Course: Recombinant Proteins, Lecture (1506-431)

Persons:

• Prof. Dr. Ralf Kölling-Paternoga (verantwortlich)

Type of Course:

lecture

In-Class Hours Per Week:

2

Contents:

In the lecture, the different steps leading to successful expression of a recombinant protein in microorganisms are presented. We closely examine the mechanisms and the control of gene transcription, mRNA translation and protein folding and other aspects connected to heterologous protein expression, like protein turnover and protein secretion. The prokaryotic model organism Escherichia coli will be contrasted with the eukaryotic model organism Saccharomyces cerevisiae. Similarities and differences in gene expression between the two will be explored. Special emphasis will be given to the practical aspects of gene expression, like gene cloning, choosing the right vector system and usage of protein tags.

Location:

Hohenheim

Remarks:

Molecular Biology of the Cell, Alberts, Wiley-VCH (2007)

Module:

• 1506-430 Recombinant Proteins (compulsory)

eLearning:

Course in ILIAS