Course: Genetic Strain Construction (1510-442)

Persons:

• Prof.Dr.-I Rudolf Hausmann (verantwortlich)
• Dr. rer. nat. Karin Moß (begleitend)
• Dr.-Ing. Marius Henkel (begleitend)

Type of Course:

practical

In-Class Hours Per Week:

4

Contents:

A prokaryotic expression system (E. coli) with a plasmid cloning vector has been suc-cessfully constructed for the heterologous protein production.
Options for different strategies have been investigated and discussed. These include:
- expression systems, promotor and induction systems, restriction endonucleas-es and respective recognition sites, genetic markers, preparation of vector, DNA-preparation, ligation, transformation, screening, molecular tags.
A laboratory work and evaluation of results have been documented in a lab journal and scientific report.

Literature:

- M. Green and J. Sambrook (2012): Molecular Cloning: A Laboratory Manual (Fourth Edition), CSH Press
- Cornel Mülhardt (2013) Der Experimentator Molekularbiologie / Genomics, Springer

Location:

Hohenheim

Remarks:

Attendance and active participation in the laboratory course is mandatory. Due to the fact that every group has full responsibility for performing their own experiment, in-lab times will be flexible but require reasonable planning on the main experimental days.

Module:

• 1510-440 Integrated Bioprocess Engineering - Upstream Processing (compulsory)

eLearning:

Course in ILIAS