Course: Bioproduction, internship (1510-422)

Persons:

• Prof.Dr.-I Rudolf Hausmann (verantwortlich)
• Dr. rer. nat. Karin Moß (begleitend)
• Dr.-Ing. Marius Henkel (begleitend)

Type of Course:

practical

In-Class Hours Per Week:

4

Contents:

Exemplary production of an heterologous protein in E.coli high cell density bioreactor cultivation
Keeping of a labjournal / protocol
Documentation and evaluation of bioreactor cultivation
Working under sterile conditions
On and off line analysis of key cultivation parameters (pO2, pH, xO2, xCO2, cell density, substrate and product concentration)
Bioreactor set-up: functions and peripherals
Independently plan and carry out operations on the bioreactor
Application of feed and induction strategies

Literature:

Henkel et al. (2015): Teaching bioprocess engineering to undergraduates: Multidisciplinary hands-on training in a one-week practical course, in: Biochemistry and Molecular Biology Education, Vol. 43, Iss. 3, pp 189–202 (http://dx.doi.org/10.1002/bmb.20860)

Location:

Hohenheim

Remarks:

Attendance and active participation in the laboratory course is mandatory. Due to the fact that every group has full responsibility for performing their own experiment, in-lab times will be flexible but require reasonable planning on the main experimental days.

Module:

• 1510-420 Integrated Bioprocess Engineering - Bioproduction (compulsory)

eLearning:

Course in ILIAS